Endotoxin Testing

Endotoxin Testing – Pyrogen Detection for Biological Safety

Even in the absence of live microbes, bacterial endotoxins—primarily lipopolysaccharides (LPS)—can introduce serious confounding variables into biological assays and animal studies. To address this, Pinnacle Compounds employs the Limulus Amebocyte Lysate (LAL) test, a highly sensitive assay derived from horseshoe crab blood that detects even trace quantities of endotoxins.

Endotoxin testing is a crucial quality control measure to ensure the safety and reliability of research materials, particularly in sensitive biological studies. Endotoxins (LPS), originating from bacterial contaminants, can interfere with experimental outcomes by triggering unwanted immune responses or introducing variability in results. To prevent these issues, endotoxin levels are carefully monitored using Limulus Amebocyte Lysate (LAL) testing, a highly sensitive assay that detects even trace amounts of endotoxins. This rigorous testing ensures that all materials meet low endotoxin thresholds, making them suitable for high-precision research models. Additionally, strict adherence to research safety protocols guarantees the integrity and reproducibility of scientific experiments, allowing researchers to work confidently with their materials.

Related Quality Testing Protocol

Our multi-layered approach to ensuring research-grade excellence.

Purity – HPLC

Mass Spectrometry (MS) verifies the molecular weight and structure of each compound. This ensures the product exactly matches its intended identity.

Safety – Endotoxin Testing (LAL)

Bioburden testing measures microbial load before sterilization. This helps prevent contamination and ensures a clean, controlled research environment.

Heavy Metals Testing

Using ICP-MS, we screen for heavy metals like lead and mercury. This ensures our compounds are free from toxic elements and meet safety standards.

Mass Spectrometry (MS)

This technique is particularly essential when dealing with structurally similar analogues, where minor sequence variations or stereochemical differences can alter pharmacological behavior.

Sterility Testing

To safeguard research integrity, particularly in applications involving injection, cell culture, or sensitive in vivo models, all applicable products undergo stringent sterility testing

Visual Inspection

Each finished unit undergoes dual-layer visual inspection prior to packaging. This includes human review under high-lux lighting and magnification

Dissolution Testing

This evaluation informs users of the most appropriate vehicle for their specific applications and is particularly critical for peptides that exhibit sequence-dependent solubility properties